产品说明
一般描述
TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.
应用
Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.
组分
The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.
基本信息
NACRES | NA.25 |
产品性质
等级 | for molecular biology |
形式 | solution |
质量 | 50x Concentrate |
杂质 | DNases, none detected RNases, none detected phosphatases, none detected proteases, none detected |
pH值(酸碱度) | 8.3±0.2 (25 ℃) |
密度 | 1.09 g/mL at 20 ℃ |
储存温度 | room temp |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich