产品说明
应用
Suitable as IEF-Marker for HPCE and for IEF-gels
其他说明
Mixture of 5 fluorescent IEF-Markers: pI 4.0, 5.5, 7.2. 7.6, 9.0 in 5 mM HCl:2-propanol (1:1), filtered through a 0.45 μm membrane filter.
重悬
For use in HPCE the marker mix should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at e.g. 280 nm (20 ℃). The maximum absorbances of the individual markers are between 308 and 350 nm. For fluorescence detection use an excitation wavelength of 310 nm (individual excitation maxima: 310 to 360 nm); the emission maxima of the individual markers will lie between 412 and 440 nm. For use in IEF-gels the marker mix can be loaded directly onto the gel (max. 1 μl per lane). The fluorescent bands are visualized directly at the end of focusing by UV light illumination (254 or 300 nm). During gel staining (for protein visualization) the fluorescent bands are lost.
基本信息
MDL编号 | MFCD03456831 |
NACRES | NA.26 |
产品性质
形式 | liquid |
质量水平 | 100 |
application(s) | cleaning products cosmetics flavors and fragrances food and beverages personal care |
储存温度 | 2-8℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H226 - H290 - H314 - H335 - H336 |
预防措施声明 | P210 - P233 - P234 - P280 - P303 + P361 + P353 - P305 + P351 + P338 |
危险分类 | Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B |
储存分类代码 | 3 - Flammable liquids |
WGK | WGK 1 |
闪点(F) | 73.4 °F |
闪点(C) | 23 ℃ |
个人防护装备 | Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter |
Sigma-Aldrich