产品说明
一般描述
Monoclonal Anti-Growth Factor Independence-1 (GFI1) (mouse IgG1 isotype) is derived from the hybridoma 2.5D.17 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with rat GFI1. Growth Factor Independence 1 (GFI1) is a zinc-finger transcriptional repressor, that is located on human chromosome 1p22. Gfi1 is highly expressed in thymus while Gfi1B is the predominant factor expressed in spleen, and both are expressed in bone marrow. It has a repression domain named SNAG at the N-terminal.† The protein also has a zinc finger DNA binding domain that is homologous to the domain in Gfi1B. Both bind to same consensus DNA sequence
特异性
The antibody epitope resides within the GFI1 SNAG transcriptional-repression domain (amino acids 7-26).
免疫原
rat growth factor independence-1 (GFI1).
应用
Monoclonal Anti-Growth Factor Independence-1 (GFI1) antibody may be used in:
生化/生理作用
Growth factor independence-1 (Gfi1) plays a key role in hematopoiesis along with Gfi1B proteins. Cytokines that regulate innate immunity induce Gfi1 expression. Deficiency of this protein causes thymic and peripherial T lymphopenia and severe abnormalities in pre-T-cell development in mice. Mutations in this gene cause defects in T lymphocyte function and development in humans. Gfi1 autoregulates its expression in T lymphocytes.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
分析说明
The antibody may also be used in EMSA (electrophoretic mobility shift analysis).
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02263329 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | 2.5D.17, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~55 kDa |
| species reactivity | mouse, rat, human |
| 浓度 | ~2 mg/mL |
| technique(s) | immunohistochemistry: suitable microarray: suitable western blot: 1-2 μg/mL using total cell extract of A549 cells |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q99684 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... GFI1(2672) mouse ... Gfi1(14581) rat ... Gfi1(24388) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
