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Anti-GAL4 DNA-BD antibody produced in rabbit

品牌
Sigma-Aldrich
货号
G3042
规格纯度
affinity isolated antibody, buffered aqueous solution
参考价格
5446.7 *本价格含增值税费
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数量
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产品名称:
Anti-GAL4 DNA-BD antibody produced in rabbit
产品介绍:

产品说明

一般描述

The polyclonal Anti-GAL4 DNA-BD rabbit antibody is a synthetic peptide that recognizes GAL4 DNA binding domain fusion proteins. GAL4 protein is an 881 amino acid transcription factor. It contains functionally independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a constituent of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).

特异性

Anti-GAL4 DNA-BD recognizes GAL4 DNA binding domain fusion proteins.

免疫原

synthetic peptide corresponding to the Saccharomyces cerevisiae GAL4 protein DNA binding domain (amino acids 39-52).

应用

Anti-GAL4 DNA-BD antibody produced in rabbit has been used in western blotting.

生化/生理作用

GAL4 transcription factor is involved in the induction of genes that regulate galactose metabolism in Saccharomyces cerevisiae. The yeast two hybrid system is widely used for the characterization of domains that are necessary and sufficient for the interaction of two known proteins by deletion and mutational analysis.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

储存及稳定性

For continuous use store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots at –20 ℃. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD02262929
NACRESNA.56

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
technique(s)western blot: 1-2 μg/mL using GAL4 (DBD 1-147) fusion protein
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg

Sigma-Aldrich

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