产品说明
应用
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.
其他说明
Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding
法律信息
Trizma is a registered trademark of sigma-aldrich Co. LLC
基本信息
线性分子式 | NH2C(CH2OH)3 · CH3COOH |
分子量 | 181.19 |
Beilstein | 3702918 |
EC 号 | 229-939-6 |
MDL编号 | MFCD00038951 |
PubChem化学物质编号 | 57653535 |
NACRES | NA.25 |
产品性质
产品线 | BioUltra |
质量水平 | 300 |
测定 | ≥99.0% (NT) |
形式 | crystalline powder |
杂质 | insoluble matter, passes filter test |
灼烧残渣 | ≤0.2% |
缺失 | ≤0.2% loss on drying, 20 ℃ (HV) |
pH值(酸碱度) | 6.0-7.0 (25 ℃, 0.5 M in H2O) |
溶解性 | H2O: 0.5 M at 20 ℃, clear, colorless |
痕量阴离子 | chloride (Cl-): ≤50 mg/kg sulfate (SO42-): ≤50 mg/kg |
痕量阳离子 | Al: ≤5 mg/kg As: ≤0.1 mg/kg Ba: ≤5 mg/kg Bi: ≤5 mg/kg Ca: ≤10 mg/kg Cd: ≤5 mg/kg Co: ≤5 mg/kg Cr: ≤5 mg/kg Cu: ≤5 mg/kg Fe: ≤5 mg/kg K: ≤50 mg/kg Li: ≤5 mg/kg Mg: ≤5 mg/kg Mn: ≤5 mg/kg Mo: ≤5 mg/kg Na: ≤50 mg/kg Ni: ≤5 mg/kg Pb: ≤5 mg/kg Sr: ≤5 mg/kg Zn: ≤5 mg/kg |
λ | 0.5 M in H2O |
紫外吸收 | λ: 260 nm Amax: 0.06 λ: 280 nm Amax: 0.05 |
SMILES string | CC(O)=O.NC(CO)(CO)CO |
InChI | 1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4) |
InChI key | PIEPQKCYPFFYMG-UHFFFAOYSA-N |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich