PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb

产品说明

一般描述

Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)

包装

10 tests in Plastic ampoule

应用

Immunoblotting (1:1000)

Immunohistochemistry (see comments)

警告

Toxicity: Standard Handling (A)

外形

In Dulbecco′s PBS (without Mg⁺² or Ca⁺²), 0.1% BSA, 50% glycerol, pH 7.3.

重悬

Following initial thaw, aliquot and freeze (-70°C).

分析说明

Positive Control
IFNγ-stimulated HeLa cells.

其他说明

When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Cuddihy, A.R., et al.1999. Oncogene8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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