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Cystatin C human

品牌
Sigma-Aldrich
货号
H5041
规格纯度
recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
参考价格
7541.62 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Cystatin C human
产品介绍:

产品说明

一般描述

HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.

应用

Cystatin C human has been used as a standard in western blot assay. It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).

包装

10 μg in poly bottle

生化/生理作用

Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.

外形

Lyophilized from a 0.2 μm filtered solution of 1×PBS

分析说明

The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.

法律信息

HumanKine is a registered trademark of Proteintech Group, Inc.

基本信息

MDL编号MFCD00163390
NACRESNA.77

产品性质

质量水平100
生物来源human
重组expressed in HEK 293 cells
测定≥95% (SDS-PAGE)
形式lyophilized powder
效能≤5 μM IC50
质量endotoxin tested
分子量dimer 12-13 kDa (non-glycosylated)
包装pkg of 10 μg
储存条件avoid repeated freeze/thaw cycles
technique(s)cell culture | mammalian: suitable
杂质≤1 EU/mg
UniProt登记号P01034
储存温度−20℃
Gene Informationhuman ... CYTC(1471)

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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