产品说明
一般描述
HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.
应用
Cystatin C human has been used as a standard in western blot assay. It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).
包装
10 μg in poly bottle
生化/生理作用
Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.
外形
Lyophilized from a 0.2 μm filtered solution of 1×PBS
分析说明
The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using
法律信息
HumanKine is a registered trademark of Proteintech Group, Inc.
基本信息
MDL编号 | MFCD00163390 |
NACRES | NA.77 |
产品性质
质量水平 | 100 |
生物来源 | human |
重组 | expressed in HEK 293 cells |
测定 | ≥95% (SDS-PAGE) |
形式 | lyophilized powder |
效能 | ≤5 μM IC50 |
质量 | endotoxin tested |
分子量 | dimer 12-13 kDa (non-glycosylated) |
包装 | pkg of 10 μg |
储存条件 | avoid repeated freeze/thaw cycles |
technique(s) | cell culture | mammalian: suitable |
杂质 | ≤1 EU/mg |
UniProt登记号 | P01034 |
储存温度 | −20℃ |
Gene Information | human ... CYTC(1471) |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich