产品说明
一般描述
Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins.
特异性
该抗体可识别HA-标签蛋白的天然和还原变性形式,并且可与 大肠杆菌 或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。
免疫原
与人流感病毒血凝素(HA)片段相对应的合成肽,称为HA-标签,与KLH结合
应用
Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
外形
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative.
基本信息
MDL编号 | MFCD00803873 |
NACRES | NA.56 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | TRITC conjugate |
抗体形式 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
克隆 | HA-7, monoclonal |
形式 | buffered aqueous solution |
储存条件 | protect from light |
浓度 | ~1 mg/mL |
technique(s) | direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins |
同位素/亚型 | IgG1 |
运输 | dry ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich