产品说明
一般描述
Histone Deacetylase 5 (HDAC5) belongs to the class IIa HDAC family. The localization of HDAC5 is both nuclear and cytoplasmic. HDAC5 gene is mapped to human chromosome at 17q21.31.
特异性
Anti-Histone Deacetylase 5 (HDAC5)(NA-16) recognizes human, rat, and mouse HDAC5.
免疫原
synthetic peptide corresponding to amino acid residues 384-399 of human HDAC5 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and mouse.
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit has been used in:
生化/生理作用
Histone Deacetylase 5 (HDAC5) shuttling to the cytoplasm occurs during myogenic differentiation, the nuclear export being stimulated by calcium/ calmodulin-dependent protein kinase (CaMK) phosphorylation at Ser259 and Ser498. HDAC5 activity is important for the differentiation of muscle cells by binding, through its N-terminal domain, to the myocyte enhancer factor-2 (MEF2) protein, thus repressing expression of MEF2 downstream genes.Overexpression of HDAC5 in various cancer cells suppresses their growth by induction of apoptosis in a p53-independent manner. It also plays a key role in many biological processes including the synoviocyte activation, neural regeneration and repair. HDAC5 is highly expressed in breast cancer and contributes to tumor progression.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
储存及稳定性
For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD06798461 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~124 kDa |
| species reactivity | rat, mouse, human |
| technique(s) | immunocytochemistry: 1-2 μg/mL using cultured NIH-3T3 or HeLa cells immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin-embedded human breast carcinoma section immunoprecipitation (IP): 1-2 μg using extract of 293T cells expressing recombinant mouse HDAC5 microarray: suitable western blot (chemiluminescent): 2-4 μg/mL using whole extract of human embryonal kidney 293T cells and whole extract of rat pheochromocytoma PC-12 cells |
| UniProt登记号 | Q9UQL6 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... HDAC5(10014) mouse ... Hdac5(15184) rat ... Hdac5(84580) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
