产品说明
一般描述
Luciferin 4-monooxygenase (UniProt: P08659; also known as EC: 1.13.12.7, Luciferase) is a peroxisomal enzyme that belongs to the ATP-dependent AMP-binding enzyme family. It catalyzes the production of oxyluciferin from luciferin in an ATP and magnesium dependent reaction and releases a photon of light when oxyluciferin returns to the ground state. This reaction proceeds in two steps where the first step involves adenylation of luciferin and in the second step luciferase acts as an oxygenase on adenyl-luciferin to produce oxyluciferin and carbon dioxide, the decay of oxyluciferin producing a photon of light of 562 nm. Luciferase from the firefly is commonly used in reporter assay where the total light emission is proportional to the luciferase activity of the sample.
特异性
This rabbit polyclonal antibody detects firefly luciferase.
免疫原
GST-tagged full length recombinant firefly luciferase.
Epitope: unknown
应用
Research Category
Secondary & Control Antibodies
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Luciferase in HeLa cells transfected with Luciferase expression plasmid.
Anti-Luciferase, Cat. No. AB3256-I, is a rabbit polyclonal antibody that detects firefly luciferase and has been tested for use in Immunocytochemistry and Western Blotting.
质量
Evaluated by Western Blotting in lysate from HeLa cells transfected with Luciferase expression plasmid.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Luciferase in HeLa cells transfected with luciferase expression plasmid.
目标描述
~58 kDa observed; 60.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity (predicted by homology) | all |
包装 | antibody small pack of 25 μL |
technique(s) | immunocytochemistry: suitable western blot: suitable |
同位素/亚型 | IgG |
UniProt登记号 | P08659 |
运输 | ambient |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich