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Anti-OTX2 Antibody

品牌
Sigma-Aldrich
货号
AB9566-I
规格纯度
from rabbit, purified by affinity chromatography
参考价格
6022.61 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti-OTX2 Antibody
OTX2, Homeobox protein OTX2, Orthodenticle homolog 2
产品介绍:

产品说明

一般描述

Homeobox protein OTX2 (UniProt P32243; also known as Orthodenticle homolog 2) is encoded by the OTX2 (also known as CPHD6, MCOPS5) gene (Gene ID 5015) in human. OTX2 is a paired-type homeobox transcription factor that controls the expression of target genes by binding to regulatory regions containing the YTAATNN motifs. Studies conducted in mice shows developmental stage-dependent expression pattern. OTX2 is widely expressed in the epiblast, but becomes progressively regionalised during gastrulation to an anterior domain of the ectoderm (anterior neuroectoderm, ANE) that contains the precursor cells of the prosencephalon and the mesencephalon of the embryonic brain. The precursor cells for the anterior visceral endoderm (AVE) fail to translocate from the distal site to the anterior region of the pre-gastrulation stage Otx2-knockout embryos. The absence of the AVE, in turn, causes the loss of the source of morphogenetic signals for inducing neural differentiation of the ANE. In addtion, OTX2 is shown to be essential in the anterior mesendoderm (AME) for the induction of anterior neural tissues and head formation. OTX2 is reported to mediate the transcriptions of Dkk1 and Lhx1, loss of Otx2 in the AME results in the downregulation of Dkk1 and Lhx1.

特异性

Expected to react with both spliced isoforms, as well as all five polymorphic forms of human OTX2 reported by UniProt (P32243). The immunogen sequence is about 78% conserved in OTX1, this polyclonal antibody may therefore recognize OTX1. However, OTX1 cross-reactivity has not been directly tested and confirmed. OTX2 is expressed earlier than OTX1 and therefore this antibody can be used to identify OTX2 during development.

免疫原

Epitope: Internal region
KLH-conjugated linear peptide corresponding to the internal region of human OTX2.

应用

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected OTX2 in human retina tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

质量

Evaluated by Western Blotting in Y79 human retinoblastoma cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected OTX2 in 10 µg of Y79 human retinoblastoma cell lysate.

目标描述

~35 kDa observed

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman
species reactivity (predicted by homology)rat (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)immunohistochemistry: suitable
western blot: suitable
NCBI登记号NP_758840
UniProt登记号P32243
Gene Informationhuman ... OTX2(5015)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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