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Anti-FcεRI α subunit Antibody

Anti-FcεRI α subunit Antibody

品牌
Sigma-Aldrich
货号
06-725-I
规格纯度
serum, from rabbit
参考价格
5520.38 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-FcεRI α subunit Antibody
High affinity immunoglobulin epsilon receptor subunit alpha, Fc-epsilon RI-alpha, FcERI, IgE Fc receptor subunit alpha
产品介绍:

产品说明

一般描述

The allergic reaction is mediated through the IgE high affinity receptor (FcεRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FcεRI receptor complexes causes the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FcεRI receptor is a tetrameric complex, consisting of an a-chain, b-chain and a dimeric g-chain. The a-chain is primarily involved with the binding of IgE, but the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FcεRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.

特异性

This antibody recognizes the extracellular domain of FcεRI α subunit.

免疫原

GST-tagged recombinant protein corresponding to the extracellular domain of human FcεRI α subunit.
Epitope: Extracellular domain

应用

Research Sub Category
Inflammation & Autoimmune Mechanisms
Western Blot Analysis: A 1:5,000 dilution from a representative lot detected FcεRI α subunit in 10 µg of human spleen tissue lysate.

Immunoprecipitation Analysis: A 1:50 dilution from a representative lot immunoprecipitated FcεRI α subunit from human spleen whole lysate.

Flow Cytometry Analysis: A 1:200 dilution from a representative lot detected FcεRI α subunit in RBL cells.
Detect FcεRI α subunit using this Anti-FcεRI α subunit Antibody validated for use in Western Blotting, Immunoprecipitation, ICC.
Research Category
Inflammation & Immunology

质量

Evaluated by Western Blot in RBL cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected FcεRI α subunit in 10 µg of RBL cell lysate.

目标描述

~57 kDa observed

联系

Replaces: 06-725

外形

Unpurified
Rabbit polyclonal serum containing 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
RBL cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivityhuman
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号NP_001992
UniProt登记号P12319
运输wet ice
Gene Informationhuman ... FCER1A(2205)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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