Anti-Beta Pix, SH3 Domain Antibody

产品说明

一般描述

Beta-Pix (ARHGEF7, βPix, COOL) is a Rho guanine nucleotide exchange factor (GEF). Beta-Pix is a p21-activated protein kinase (PAK)-interacting exchange factor (Pix, COOL, p85SPR) that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. The variants of Beta-Pix isoforms exhibit different tissue distributions. Beta-Pix-a is expressed ubiquitously in all the tissues examined, and other isoforms are expressed mainly in the central nervous system. PAK, a Ser/Thr kinase, is an important mediator of Rho family activity interacts with Beta-Pix at the SH3 domain of Beta-Pix though its proline rich region. This interaction is thought to affect the GTP hydrolysis rate of Rac and Cdc42.

免疫原

Epitope: SH3 domain
GST-tagged recombinant protein corresponding to the SH3 domain of human Beta-Pix, SH3 Domain.

应用

Western Blotting Analysis: 1 µg/mL from a representative lot detected Beta-Pix, SH3 Domain in 10 µg of PC-12 cell lysate.
Detect Beta Pix using this Anti-Beta Pix antibody, SH3 Domain validated for use in western blotting.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Beta-Pix, SH3 Domain in 10 µg of Jurkat cell lysate.

目标描述

~80 kDa observed

联系

Replaces: 07-1450

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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