产品说明
应用
Interleukin-12 (IL-12) from mouse has been used to study the effect of IL-12 on Th1 (type 1 T helper) immunity in Mongolian gerbils (Meriones unguiculatus) infected with Sporothrix schenckii. It has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.
生化/生理作用
Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
基本信息
| MDL编号 | MFCD00212975 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 重组 | expressed in baculovirus infected Sf21 cells |
| 测定 | ≥97% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 效能 | 0.02-0.4 ng/mL EC50 |
| 分子量 | glycoprotein 70 kDa |
| 包装 | pkg of 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| UniProt登记号 | P43431, P43432 |
| 储存温度 | −20℃ |
| Gene Information | mouse ... Il12a(16159), Il12b(16160) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
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