产品说明
一般描述
Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.
特异性
Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%
应用
Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Research Sub Category
Obesity
Metabolic Disorders
Research Category
Metabolism
Used to detect/quantify: Ghrelin (Total)
其他说明
Please contact Technical Service for linearity of dilution.
免责声明
For research use only. Not for use in diagnostic procedures.
基本信息
| eCl@ss | 32161000 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 100 |
| species reactivity | rat, mouse |
| assay range | accuracy: 80-103% (Serum: Active Ghrelin) accuracy: 86-102% (Plasma: Active Ghrelin) accuracy: 92-103% (Serum: Des-octanoyl Ghrelin) accuracy: 95-107% (Plasma: Des-octanoyl Ghrelin) inter-assay cv: 1.8-4.5% intra-assay cv: 0.7-1.3% (Serum) inter-assay cv: 2.0-3.2% intra-assay cv: 1.1-1.7% (Plasma) |
| technique(s) | ELISA: suitable |
| UniProt登记号 | Q9EQX0 (mouse) Q9QYH7 (rat) Q9UBU3 |
| detection method | colorimetric fluorometric |
安全信息
| 象形图 |    |
| 警示用语： | Danger |
| 危险声明 | H290 - H302 + H332 - H311 - H315 - H317 - H319 - H335 - H411 |
| 预防措施声明 | P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 |
| 危险分类 | Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 3 |
Sigma-Aldrich
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