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Anti-EGFR (Ab-3) Mouse mAb (225)

品牌
Sigma-Aldrich
货号
GR13L
规格纯度
lyophilized, clone 225, Calbiochem®
参考价格
6526.19 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-EGFR (Ab-3) Mouse mAb (225)
Anti-EGF-R
产品介绍:

产品说明

一般描述

Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.
Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.

免疫原

partially purified, human EGFR from A431 cells
Human

包装

100 μg in Plastic ampoule

应用

Immunoprecipitation (1-2 µg per sample)

Neutralization Studies (2 µg/ml, see comments and application references)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.

重悬

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

分析说明

Positive Control
A431 cells
Negative Control
HL-60 cells

其他说明

Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆225, monoclonal
形式lyophilized
不包含 preservative
species reactivityhuman
should not react with mouse, rat
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
同位素/亚型IgG1
运输ambient
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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