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Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

品牌
Sigma-Aldrich
货号
GR15L
规格纯度
lyophilized, clone EGFR.1, Calbiochem®
参考价格
6257.34 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)
Anti-Epidermal Growth Factor Receptor
产品介绍:

产品说明

一般描述

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies.
Recognizes the ~170 kDa EGF receptor protein in A431 cells.

免疫原

partially purified, human EGFR from A431 cells
Human

包装

100 μg in Plastic ampoule

应用


Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; see application references)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from a volatile buffer, 100 µg BSA.

重悬

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

分析说明

Negative Control
HL-60 cells
Positive Control
A-431 cells

其他说明

Stains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆EGFR.1, monoclonal
形式lyophilized
不包含 preservative
species reactivityhuman
should not react with rat, mouse
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
同位素/亚型IgG2b
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3

Sigma-Aldrich

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