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Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2)

品牌
Sigma-Aldrich
货号
GR32L
规格纯度
lyophilized, clone BuGR2, Calbiochem®
参考价格
7365.52 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2)
产品介绍:

产品说明

一般描述

Recognizes the ~94-97 kDa glucocorticoid receptor in mouse liver tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-AgX-653 myeloma cells (see application references). Recognizes the ~94-97 kDa glucocorticoid receptor.
Anti-Glucocorticoid Receptor (Ab-2), mouse monoclonal, clone BuGR2, recognizes ~94-97 kDa glucocorticoid receptor in mouse liver. It is validated for WB, ICC, IP & IHC (Frozen & paraffin sections).

免疫原

Rat
partially purified, rat glucocorticoid receptor

包装

100 μg in Plastic ampoule

应用


Frozen Sections (5 g/ml)
Immunoblotting (5 g/ml)
Immunocytochemistry (5 g/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 g/ml)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from PBS, pH 7.2.

重悬

Resuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

分析说明

Positive Control
mouse liver extract

其他说明

Does not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA94, 1521.
Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev.18 306.
Schumacher, M., et al. 1996. Dev. Neurosci.18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci.18, 36.
Weigel, N.L. 1996. Biochem J.319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci.743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆BuGR2, monoclonal
形式lyophilized
包含≤0.1% sodium azide as preservative
species reactivityrat, mouse, guinea pig, sheep, rabbit
should not react with avian, amphibian, primate
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
同位素/亚型IgG2a
运输ambient
储存温度−20℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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