Anti-Neuropeptide Y Rabbit pAb

产品说明

一般描述

Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes neuropeptide Y.
This Anti-Neuropeptide Y Rabbit pAb is validated for use in Frozen Sections for the detection of Neuropeptide Y.
Recognizes neuropeptide Y in cortex, caudate, putamen, and paraventricular nucleus.

免疫原

porcine neuropeptide Y

包装

100 μL in Plastic ampoule

应用

Frozen Sections (1:1000-1:2000 for free-floating, PAP detection)

警告

Toxicity: Standard Handling (A)

重悬

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

分析说明

Positive Control
Paraventricular nucleus, cortex, or caudate putamen

其他说明

Antibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10^-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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安全信息