产品说明
一般描述
Recognizes vasopressin in rat hypothalamus.
This Anti-Vasopressin Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Vasopressin.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.
免疫原
Rat
rat arginine vasopressin
包装
100 μL in Plastic ampoule
应用
Frozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
Immunofluorescence (1:200-1:400)
警告
Toxicity: Standard Handling (A)
外形
Undiluted serum.
重悬
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
分析说明
Positive Control
Rat hypothalamus
其他说明
Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Antibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | lyophilized |
包含 | ≤0.1% sodium azide as preservative |
species reactivity | rat |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze |
同位素/亚型 | IgG |
运输 | ambient |
储存温度 | −20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich