Anti-NIPP-1 Antibody

产品说明

一般描述

Also known as ARD1 (Activator of RNA decay), NIPP-1 (nuclear inhibitor of protein phosphatase 1) is a ubiquitously expressed multifunctional nuclear scaffold protein of 39 kDa, which plays an important role in integrating transcription, RNA processing, and cell cycle progression. NIPP-1 serves as a signaling hub in the nucleus. Additionally, NIPP-1 contains at least two binding sites for PP1 and is complexed to about one-third of the nuclear pool of PP1. Normally, the NIPP-1 - PP1 complex is inactive, but can be activated following phosphorylation of NIPP-1 with PKA, CFK2, or Src family kinases. NIPP-1 also interacts directly with nucleic acids and components of polycomb repressive complexe 2 (PRC2) and functions as a PRC2-dependent transcriptional repressor.

特异性

This antibody should recognize the alpha and beta isoforms of NIPP-1.

免疫原

GST-tagged recombinant protein corresponding to human NIPP-1.

应用

This NIPP-1 antibody is validated for use in WB & ICC for the detection of the NIPP-1 protein.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NIPP-1 in Raji cells.

质量

Evaluated by Western Blot Analysis in Raji nuclear extract.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected NIPP-1 in 10 µg of Raji nuclear extract.

目标描述

~42 kDa observed. Three isoforms at 39 kDa (alpha isoform), 23 kDa (beta isoform), and 13 kDa (gamma isoform) are known to exist. An uncharacterized band may be observed at ~62 kDa in some nuclear extracts.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Raji nuclear extract

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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