产品说明
一般描述
Product Source: expressed individually by baculovirus in Sf21 insect cells
C-terminal His6-tagged human full length cdk1, and N-terminal GST-tagged human full length cyclin B
生化/生理作用
Protein Target: CDK1/cyclinB
Target Sub-Family: CMGC
包装
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-450M when ordering the 250 µg size.
质量
routinely evaluated by phosphorylation of the Histone H1 substrate
外形
Purified using Ni2+/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose® and Ni2+/NTA-agarose
储存及稳定性
6 months at -70°C
其他说明
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法律信息
Sepharose is a registered trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160405 |
NACRES | NA.26 |
产品性质
质量水平 | 100 |
生物来源 | human |
分子量 | Mw 35 kDa (cdk1) Mw 75 kDa (cyclin B) |
manufacturer/tradename | Upstate® |
technique(s) | activity assay: suitable (kinase) |
NCBI登记号 | NM_001786.2 NM_033379.2 |
UniProt登记号 | P06493 |
Sigma-Aldrich