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Monoclonal Anti-MAT-Tag® antibody produced in mouse

品牌
Sigma-Aldrich
货号
M6693
规格纯度
purified immunoglobulin, ~2 mg/mL, clone MAT1-87
参考价格
5012.7 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Monoclonal Anti-MAT-Tag® antibody produced in mouse
产品介绍:

产品说明

一般描述

Monoclonal Anti-Mat-Tag antibody was used in one-step purification of histidine-tagged fusion proteins under high pressure and high flow conditions. It is used in the characterization of the molecular architecture of human caveolin-3 and interaction with the skeletal muscle ryanodine receptor.
Monoclonal Anti-MAT (mouse IgG2a isotype) is derived from the MAT1-87 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic MAT peptide HNHRHKHGGGC conjugated to KLH.

特异性

Monoclonal Anti-MAT recognizes the seven amino acid MAT epitope sequence within either N or C terminal MAT-tagged fusion proteins.

免疫原

synthetic MAT peptide HNHRHKHGGGC.

应用

Monoclonal Anti-MAT-Tag® antibody produced in mouse has been used in:

  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry


The MAT tag is a seven amino acid peptide tag that binds to transition metals such as Nickel and Cobalt. This tag, which consists of the sequence HNHRHKH, allows the purification of MAT fusion proteins using immobilized metal affinity chromatography (IMAC) such as the highly selective HIS-Select® Nickel Affinity Gel. The MAT tag sequence can be incorporated into both prokaryotic and eukaryotic expression vectors.

外形

Supplied as a solution in 0.01M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

法律信息

HIS-Select is a registered trademark of sigma-aldrich Co. LLC
MAT-Tag is a registered trademark of sigma-aldrich Co. LLC
TagCFP is a trademark of Evrogen Co.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD06798711
NACRESNA.56

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆MAT1-87, monoclonal
浓度~2 mg/mL
technique(s)immunoprecipitation (IP): 5-10 μg
western blot: 1-2 μg/mL
同位素/亚型IgG2a
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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