产品说明
一般描述
FAM65B (family with sequence similarity 65, member B), also known as chromosome 6 open reading frame 32 (C6ORF32), is mapped to human chromosome 6p22.3. FAM65B is known to be expressed during embryonic and postnatal development stages in cochlea. The encoded protein is found to concentrate in the plasma membranes of the stereocilia of inner and outer hair cells of the inner ear.
免疫原
C6orf32 (AAH01232.1, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MLVGSQSFSPGGPNGIIRSQSFAGFSGLQERRSRCNSFIENSSALKKPQAKLKKMHNLGHKNNNPPKEPQPKRVEEVYRALKNGLDEYLEVHQTELDKLTAQLKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEAYCIQRRLQDGASKMKQAFATSPASKAARESLTEINRSFKEYTENMCTIEVELENLLGEFSIKMKGLAGFARLCPGDQYEIFMKYGRQRWKLKGKIEVNGKQSWDGEETVFLPLIVGFISIKVTELKGLATHILVGSVTCETKELFAARPQVVAVDINDLGTIKLNLEITWYPFDVEDMTASSGAGNKAAALQRRMSMYSQGTPETPTFKDHSFFSNLPDDIFENGKAAEEKMPLSLSFSDLPNGDCALTSHSTGSPSNSTNPEITITPAEFNLSSLASQNEGMDDTSSASSRNSLGEGQEPKSHLKEEDPEEPRKPASAPSEACRRQSSGAGAEHLFLENDVAEALLQESEEASELKPVELDTSEGNITKQLVKRLTSAEVPMATDRLLSEGSVGGESEGCRSFLDGSLEDAFNGLLLALEPHKEQYKEFQDLNQEVMNLDDILKK
生化/生理作用
FAM65B (family with sequence similarity 65, member B) is recognized as a Ras homolog gene family, member A (RHOA) inhibitor and is involved in polarization. FAM65B regulates RhoA activity with the help of forkhead box O transcription factors. It binds to RhoA and blocks the GTP loading to RhoA. FAM65B deficiency in neutrophils leads to an increase in RhoA activity and causes defects in chemotaxis directionality and adhesion to endothelial cells under flow. It is involved in chemokine-stimulated migration and adhesion of Jurkat cells. Phosphorylation stabilizes FAM65B. FAM65B is a component of the inner ear and is involved in the process of hearing. Mutation in the gene is known to be associated with hearing loss in humans. FAM65B is associated with myotube formation and in the regulation of cell adhesion, polarization, and migration. Overexpression of the protein in myogenic and non-myogenic cells results in the formation of cellular protrusions. Hence, FAM65B might be involved in myoblast migration or cytoskeletal protein rearrangements that accompany myogenic differentiation.
外形
Solution in phosphate buffered saline, pH 7.4
法律信息
GenBank is a registered trademark of United States Department of Health and Human Services
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 2E6-1B10, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | human |
| technique(s) | indirect ELISA: suitable western blot: 1-5 μg/mL |
| 同位素/亚型 | IgG2bκ |
| GenBank® accession no. | BC001232 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... FAM65B(9750) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
