Anti-proSAAS

产品说明

一般描述

ProSAAS (UniProt: Q9QXV0; also known as IA-4, Proprotein convertase subtilisin/kexin type 1 inhibitor, Proprotein convertase 1 inhibitor, pro-SAAS) is encoded by the Pcsk1n gene (Gene ID: 30052) in murine species. ProSAAS is a small secretory protein that is primarily expressed in neural and neuroendocrine tissues and acts as an anti-aggregant chaperone. Its presence appears to be restricted only to vertebrates. Mammalian proSAAS does not undergo either glycosylation or phosphorylation, but undergoes proteolytic cleavage at several pairs of basic residues that leaves its core region intact. ProSAAS is known to interact with proprotein convertase subtilisin/kexin type 1 (PCSK1) via the C-terminal inhibitory domain (aa 219-258). In the neural tissues proSAAS exhibits neuroprotective effects and is shown to block alpha-synuclein-induced cytotoxicity in cultures of dopaminergic neurons and in SH-SY5Y cells. In vitro studies have shown that proSAAS can also prevent the fibrillation of amyloid beta 1-42 peptide in a dose-dependent manner and residues 97-180 are sufficient for its anti-aggregation function against amyloid peptides. Inclusion of recombinant proSAAS in the medium of Neuro2a cells is shown to block the neurocytotoxic effect of amyloid peptide 1-42.

特异性

This rabbit polyclonal antibody detects proSAAS in human, mouse, and rat cells. It targets an epitope within the N-terminal half.

免疫原

His-tagged recombinant fragment corresponding to first 180 amino acids from murine ProSAAS.

应用

Anti-proSAAS Antibody, Cat. No. ABN2268, is a highly specific rabbit polyclonal antibody that targets ProSAAS and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected proSAAS in AtT-20 cells. (Courtesy of Dr. Iris Lindberg @ University of Maryland).

Immunofluorescence Analysis: A representative lot detected proSAAS in Immunofluorescence applications (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15; Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30; Guest, P.C., et. al. (2002). J Endocrinol. 175(3):637-47).

Western Blotting Analysis: A representative lot detected proSAAS in A immunoprecipitated from mouse brain lysates (Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30). Notes: Treating blots with 1% glutaraldehyde in PBS for 30 min prior to blocking significantly increased the proSAAS signal and reduces the background.

Immunocytochemistry Analysis: A representative lot detected proSAAS in SH-SY5Y cells (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15).

质量

Evaluated by Western Blotting in His-tagged mouse proSAAS recombinant protein.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His-tagged mouse proSAAS recombinant protein. Note: For most biological samples a range of 1:1000 for Western blotting and 1:500 for IHC is recommended.

目标描述

~19 kDa observed; 27.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Unpurified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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