产品说明
一般描述
The Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems.In mammals, four Notch genes were identified (Notch1-4) in a wide variety of cells and play a crucial role in differentiation and development. Notch proteins contain three main domains: an extracellular domain containing tandem epidermal growth factor repeats, and three lin-12/Notch repeats, a transmembrane domain, and an intracellular domainthat contains six cdc1-/SW16/ankyrin repeats, nuclear localization signals, and a C-terminal OPA/PEST region.
Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.
特异性
Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.
免疫原
recombinant protein containing the cdc10-NCR region of mouse Notch1.
应用
Anti-Notch1 antibody, mouse monoclonal has been used in:
包装
200 μL in glass insert
生化/生理作用
NOTCH is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism. Fibronectin functions both as a regulator of cellular processes and as an important scaffolding protein to maintain and direct tissue organization and ECM composition.Fibronectin is suggested to enhance cell adhesion, spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
MDL编号 | MFCD03455659 |
NACRES | NA.41 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | unconjugated |
抗体形式 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
克隆 | mN1A, monoclonal |
形式 | buffered aqueous solution |
species reactivity | mouse, human |
technique(s) | immunocytochemistry: suitable indirect ELISA: suitable microarray: suitable western blot: 1-2 μg/mL using extracts of cells stably transfected with a truncated Notch1 (32D-mN1-ICDOP that expresses the intracellular mouse Notch1 molecule-65 kDa). |
同位素/亚型 | IgG1 |
UniProt登记号 | P46531 |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | human ... NOTCH1(4851) mouse ... Notch1(18128) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich