产品说明
一般描述
REK from Cell Applications, Inc. provide a suitable model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology. It was shown that TGFβ1 treatment decreased c-Myc mRNA in REK which, taken together with the TGFβ1-induced growth arrest exhibited by keratinocytes and opposite results obtained in other cells, suggests a correlation between c-Myc mRNA expression and the mitogenic response (Nakai, 2008). Additionally, REK were used to demonstrate that pathological increases in keratinocyte sodium channels Nav1.1, Nav1.6, and Nav1.8 expression may contribute to pain by increasing epidermal ATP release, resulting in excessive activation of P2X receptors on primary sensory axons (Zhao, 2008). REK were also used to demonstrate that laminin 332 deposition is inhibited by ionizing radiation and, in combination with slower keratinocyte migration, can contribute to the delayed wound healing of irradiated skin (Jourdan, 2011).
细胞系来源
Skin
应用
Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response.
组分
Rat Keratinocyte Basal Medium that contains 10% FBS and 10% DMSO
质量
Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
制备说明
传代培养常规
Please refer to the RDF Culture Protocol.
基本信息
NACRES | NA.81 |
产品性质
生物来源 | rat skin (Sprague Dawley) |
质量水平 | 100 |
包装 | pkg of 500,000 cells |
生长模式 | Adherent |
形态学 | Epidermal |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | allergies; cancer |
运输 | dry ice |
储存温度 | −196℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich