产品说明
应用
Oncostatin M from mouse has been used in the preparation of culture medium for primary culture of mouse fetal hepatoblasts (MFHs).
生化/生理作用
Oncostatin M, LIF, G-CSF, IL-6, and ciliary neurotrophic factor (CNTF) are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components. Oncostatin M is a growth-regulating cytokine, affecting a number of tumor and normal cells. This material was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. It acts synergistically with TGF β1 to inhibit the proliferation of tumor cells like A375 melanoma cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. Oncostatin M is secreted by macrophages and activated T lymphocytes.
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 1.25 mg bovine serum albumin.
分析说明
The biological activity of recombinant mouse OSM was measured by its ability to stimulate 3H-thymidine incorporation in quiescent NIH/3T3 cells.
基本信息
| MDL编号 | MFCD01094784 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 重组 | expressed in E. coli |
| 产品线 | BioReagent |
| 测定 | ≥97% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 效能 | 0.25-1 ng/mL EC50 |
| 分子量 | 21 kDa |
| 包装 | pkg of 25 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| UniProt登记号 | P53347 |
| 储存温度 | −20℃ |
| Gene Information | mouse ... Osm(18413) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
