产品说明
一般描述
Oncostatin M (OSM) is produced by activated T cells, monocytes and Kaposi′s sarcoma cells. OSM shares several structural and functional characteristics with leukemia inhibitory factor (LIF), interleukin-6 (IL-6), and ciliary neurotrophic factor (CNTF).
应用
Human oncostatin-M has been used to stimulate bovine full-depth cartilage explants. It has also been used to culture embryonic stem cells in standard minimum essential medium-α.
生化/生理作用
Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. It can exert both stimulatory and inhibitory effects on cell proliferation. OSM stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi′s sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release [e.g. interleukin-6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte-colony-stimulating factor (G-CSF)] from endothelial cells.
Oncostatin M (OSM), LIF, G-CSF, IL-6, and CNTF are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components. OSM is a growth-regulating cytokine, affecting a number of tumor and normal cells. This material was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. It acts synergistically with TGF β1 to inhibit the proliferation of tumor cells like A375 melanoma cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. OSM is secreted by macrophages and activated T lymphocytes.
外形
Lyophilized from a 0.2 μm filtered solution in 10 mM acetic acid, containing 50 μg of bovine serum albumin per 1 μg of cytokine.
分析说明
The proliferative activity of human oncostatin M is tested in culture by using a human erythroleukemic cell line, TF-1.
基本信息
| EC 号 | 200-835-2 |
| MDL编号 | MFCD00166731 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 产品线 | BioReagent |
| 测定 | ≥97% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 效能 | ≤1.000 ng/mL ED50 |
| 分子量 | ~22 kDa |
| 包装 | pkg of 10 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| 颜色 | white |
| 溶解性 | soluble 0.100 mL, clear, colorless (Solvent-PBS + 0.1% BSA) |
| UniProt登记号 | P13725 |
| 储存温度 | −20℃ |
| Gene Information | human ... OSM(5008) |
安全信息
| 储存分类代码 | 4.1B - Flammable solid hazardous materials |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter |
Sigma-Aldrich
m.cnreagent.com
