产品说明
一般描述
Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.
应用
Anti-Osteopontin antibody produced in rabbit has been used for ELISA.
生化/生理作用
A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
分析说明
The biological activity is measured by its ability to mediate 293 cell adhesion.
基本信息
| MDL编号 | MFCD03100698 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 100 |
| 生物来源 | human |
| 重组 | expressed in NSO cells |
| 无菌性 | sterile |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 分子量 | 60-65 kDa by SDS-PAGE (reducing) |
| 包装 | pkg of 50 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| UniProt登记号 | P10451 |
| 运输 | ambient |
| 储存温度 | −20℃ |
| Gene Information | human ... SPP1(6696) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
