产品说明
一般描述
Programmed cell death 4 (PDCD4) also known as nuclear antigen H731-like, Protein 197/15a, neoplastic transformation inhibitor protein), is a tumor suppressor protein that is lost in progressed carcinomas of lung, breast, colon and prostate.
应用
Anti-phospho-PDCD4 (pSer67) antibody produced in rabbit has been used in immunoblotting.
生化/生理作用
Programmed cell death 4 (PDCD4) suppresses translation initiation by specifically inhibiting the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex and by competing with eIF4G, a second component of the translation initiation complex, for binding to eIF4A. In response to mitogens, PDCD4 was rapidly phosphorylated on Ser67 by the protein kinase S6K1 and subsequently degraded via the ubiquitin ligase SCF-β (TRCP). It has been shown that the protein is specifically phosphorylated on Ser67 and Ser457 by Akt, both in vitro and in vivo. This phosphorylation causes nuclear translocation of PDCD4.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~51 kDa |
| species reactivity | mouse (predicted), human, rat (predicted) |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells starved for 48 hours and then treated with 20% FCS western blot: 1-2 μg/mL using lystes of HEK-293T cells starved for 48 hours and then treated with 20% FCS |
| UniProt登记号 | Q53EL6 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PDCD4(27250), SELP(6403) mouse ... Pdcd4(18569) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
