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Anti-Dopamine Transporter Antibody

品牌
Sigma-Aldrich
货号
AB1591P
规格纯度
Chemicon®, from rabbit
参考价格
6022.61 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Dopamine Transporter Antibody
DAT
产品介绍:

产品说明

一般描述

Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson′s disease, Tourette′s disease, schizophrenia, and addiction. It is a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm.

特异性

Recognizes Dopamine transporter (DAT). By Western blot the antibody recognizes a broad 80 kDa band in rat striatum (Lew et al. 1992; Freed et al. 1995; Vaughan et al. 1996) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.

免疫原

An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (Kilty et al. 1991; Giros et al. 1991; Shimada et al. 1991; Kitayma 1992). This region is presumably the cytoplasmic domain.

应用

Western blot: 1-10 μg/mL using ECL. An antibody raised against a similar peptide has been shown to react with a broad 75-80 kDa band in rat striatum (Hastrup et al., 2001) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.

Immunoprecipitation: 2-10 μg of antibody (Chang et al., 2001)

Immunohistochemistry: 1:250-1:500 on frozen sections of tissues fixed with 4% paraformaldehyde (Chang et al., 2001; Ourednik, et al., 2002). 0.05% trypsin for 10-15min has been found effective in exposing the epitope in some preparations.

ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide (Catalog Number AG296) per well.

Optimal working dilutions must be determined by end user.
Detect Dopamine Transporter using this Anti-Dopamine Transporter Antibody validated for use in ELISA, IH, IP & WB.

目标描述

80 kDa

外形

ImmunoAffinity Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, rat
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号NM_001044.3
UniProt登记号Q01959
运输dry ice
Gene Informationhuman ... SLC6A3(6531)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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