Anti-Mouse Collagen Type I Antibody

产品说明

一般描述

Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. It is one of the long, fibrous structural proteins whose functions are quite different from those of globular proteins such as enzymes; tough bundles of collagen called collagen fibers are a major component of the extracellular matrix that supports most tissues and gives cells structure from the outside, but collagen is also found inside certain cells. Collagen has great tensile strength, and is the main component of fascia, cartilage, ligaments, tendons, bone and teeth. Along with soft keratin, it is responsible for skin strength and elasticity, and its degradation leads to wrinkles that accompany aging. It strengthens blood vessels and plays a role in tissue development. It is present in the cornea and lens of the eye in crystalline form. Collagen occurs in many places throughout the body. There are 12 types of collagen described in literature. Collagen Type 1: This is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone. Collagen Type 4: It is present in basal lamina and the eye lens. Also serves as part of the filtration system in capillaries and the glomeruli of nephron in the kidney.
Collagen Type I extracted and purified from mouse skin. Antibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.

特异性

Antibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.
Recognizes mouse Collagen Type I.

免疫原

Collagen Type I extracted and purified from mouse skin.

应用

Research Category
Cell Structure
Anti-Mouse Collagen Type I Antibody detects level of Mouse Collagen Type I and has been published in over 20 citations and validated for use in ELISA, IH, RIA and WB.
Western Blot Analysis:
1:500 dilution of a previous lot detected Collagen Type I on 10 μg of Mouse Liver lysate.

Immunohistochemistry:
1:40 dilution of a previous lot for immunofluorescent staining of frozen mouse skin and liver tissues.

RIA:
A 1:200 dilution of a previous lot was used in RIA.

ELISA:
A previous lot of this antibody was used in ELISA
Research Sub Category
ECM Proteins

目标描述

MW of Collagen Type I precursor: 140-210 kDa MW of mature Collagen Type I: 70-90 kDa

联系

Replaces: AB765

外形

Purified rabbit polyclonal serum in buffer containing 0.01 M phosphate, 0.09 M NaCl, pH 7.2. No preservatives.
Format: Purified
Protein A purified

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.

分析说明

Control
Mouse skin and mouse liver tissues

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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