产品说明
特异性
Recognizes rat Sodium, Potassium, Chloride Cotransporter 2 (NKCC2, BSC1, CCC2). The immunogen shows no significant sequence homology with NKCC1 or TSC.
SPECIES REACTIVITIES: The immunogen sequence is 100% conserved in mouse, 87% in human and rabbit. Reactivity with other species has not been confirmed.
免疫原
A 15 amino acid peptide sequence near the C-terminus of rat NKCC2 (Haas et al. 1987; Gamba et al. 1994; Simon et al. 1996; Mount et al. 1995; Igarashi et al. 1995; Mount et al. 1998). The immunogen peptide is available (catalog number AG205) for use in pre-absortion controls.
应用
Anti-Na+/K+/Cl-Cotransporter 2 Antibody detects level of Na+/K+/Cl-Cotransporter 2 & has been published & validated for use in ELISA & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1-10 μg/mL using ECL. Full length human NKCC2 is ~150 kDa.
ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide per well.
Optimal working dilutions must be determined by the end user.
外形
Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | rat, mouse |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable western blot: suitable |
NCBI登记号 | NM_000338.2 |
UniProt登记号 | Q13621 |
运输 | dry ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich