产品说明
一般描述
Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).
Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies.
特异性
Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.
免疫原
Enriched fraction of rabbit IgG light chain.
应用
Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in:
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | peroxidase conjugate |
| 抗体形式 | purified immunoglobulin |
| antibody product type | secondary antibodies |
| 克隆 | RabT-50, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | rabbit |
| should not react with | human, pig, rat, goat, monkey, bovine, horse, chicken, cat |
| 浓度 | 2.5-5.0 mg/mL (antibody) |
| technique(s) | immunocytochemistry: suitable indirect ELISA: suitable western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody. |
| 同位素/亚型 | IgG1 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H317 |
| 预防措施声明 | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| 危险分类 | Skin Sens. 1 |
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
