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Monoclonal Anti-RAL-A antibody produced in mouse

品牌
Sigma-Aldrich
货号
R8529
规格纯度
~2 mg/mL, clone RalA5F9.2, purified immunoglobulin, buffered aqueous solution
参考价格
4731.57 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Monoclonal Anti-RAL-A antibody produced in mouse
产品介绍:

产品说明

一般描述

Monoclonal Anti-Ral-A (mouse IgG1 isotype) is derived from the hybridoma RalA5F9.2 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. Ras-related protein Ral-A is a member of the Ras-like guanosine-5′-triphosphate (GTP) binding protein family. Ral-A protein is expressed in the islets of the pancreas, insulin-secreting cell lines and tissues during embryogenesis. The RALA gene is located on the human chromosome at 7p14.1.

特异性

Monoclonal Anti-Ral-A recognizes human, monkey, rat and mouse Ral-A, ~25 kDa.

免疫原

synthetic peptide corresponding to amino-acids 189-203 of human Ral-A, conjugated to KLH.

应用

Monoclonal Anti-RAL-A antibody produced in mouse may be used in:

  • immunoblotting
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA)

生化/生理作用

Ras-related protein Ral-A can bind guanosine triphosphate (GTP) or guanosine diphosphate (GDP). It is known to possess a low intrinsic GTPase activity. Ral-A protein is involved in regulating the insulin secretion of pancreatic β-cells. This protein is also involved in membrane trafficking, oncogenic transformation and proliferation.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

储存及稳定性

For extended storage, freeze at –20 ℃ in working aliquots. For continuous use, store at 2-8 ℃ for up to one month. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD08705597
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆RalA5F9.2, monoclonal
形式buffered aqueous solution
分子量antigen 25 kDa
species reactivitymouse, rat, human, monkey
浓度~2 mg/mL
technique(s)immunocytochemistry: 5-10 μg/mL using HeLa cells
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using rat brain extract
同位素/亚型IgG1
UniProt登记号P11233
运输dry ice
储存温度−20℃
Gene Informationhuman ... RALA(5898)
mouse ... Rala(56044)
rat ... Rala(81757)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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