Anti-Synuclein α Antibody

产品说明

一般描述

alpha-Synuclein is a small (140 amino acid) cytoplasmic protein of unclear function that is enriched in presynaptic terminals and is the precursor protein of a nonamyloid beta component of senile plaques in Alzeheimer′s Disease (AD). α-Synuclein is located predominantly in the presynaptic nerve terminals in the brain but is also found in low concentrations in all tissues except liver. Recent studies have shown that abnormal aggregation and accumulation of synaptic proteins, including α-Synuclein might be associated with plaque formation in AD and seems to be a major component of Lewy bodies in dementia with Lewy bodies (DLB). It has also been identified in the Glial Cytoplasmic Inclusions (GCIs) found in Multiple System Atrophy (MSA).

特异性

Has not been tested on other species.
Alpha-synuclein. The immunizing peptide was chosen to reduce cross-reactivity of AB5038 with β-synuclein to a minimum. Immunohistochemical and Western blot analysis of human brain indicates a high level of specificity.

免疫原

Human alpha-synuclein residue 111-131 coupled to KLH.
Epitope: a.a. 111-131

应用

Immunohistochemistry:
1:1,000-1:2,000 dilutoin of a previous lot was used on paraffin sections; 1:4,000-1:12,000 dilution of a previous lot was used on frozen sections.

Western blot:
1:1,000-1:2,000. Shows a 14-19kDa band.

Immunocytochemistry:
Previous lot used IC.

Optimal working dilutions must be determined by end user.
Anti-Synuclein Antibody, α detects level of Synuclein & has been published & validated for use in IC, IH, IH(P) & WB.

质量

Evaluated by Western Blot on Human Brain lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected Synuclein on 10 µg of human brain lysates.

目标描述

~14-19 kDa

联系

Replaces: 04-1053

外形

Rabbit polyclonal serum lyophilized, no preservatives. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any residue.
Unpurified

分析说明

Control
Human brain sections.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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