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Anti-Caspase 3 Antibody, active (cleaved) form

品牌
Sigma-Aldrich
货号
AB3623
规格纯度
Chemicon®, from rabbit
参考价格
6190.47 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Caspase 3 Antibody, active (cleaved) form
Apopain, Yama Protein, CASP3, SCA-1, procaspase3
产品介绍:

产品说明

一般描述

Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

特异性

Reactivity with other species has not been determined.
AB3623 detects only the cleaved p17 fragment and does not detect the precursor form in cells undergoing apoptosis.

免疫原

Cleavage specific synthetic peptide sequence (proprietary).
Epitope: active (cleaved) form

应用

Anti-Caspase 3 Antibody, active (cleaved) form detects level of Caspase 3 and has been published and validated for use in Immunofluorescence (IF), Immunohistochemistry (IHC), and Western Blot (WB).
Western Blot Analysis:
1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3.
Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal.

Immunohistochemistry:
1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues.

Immunohistochemistry(paraffin):
Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine
Research Sub Category
Caspases

Enzymes & Biochemistry
Research Category
Apoptosis & Cancer

Metabolism

目标描述

17 kDa

联系

Replaces: 04-440; 04-1088

外形

Purified rabbit polyclonal in buffer containing PBS, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
ImmunoAffinity Purified

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.

分析说明

Control
Tonsil and appendix tissue. Jurkat cells induced for apoptosis with recombinant soluble TRAIL and staurosporine treated mouse 3T3 cell lysates Skin/Tumor, Embryo.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human, rat
manufacturer/tradenameChemicon®
technique(s)immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI登记号NM_004346.3
UniProt登记号P42574
运输dry ice
Gene Informationhuman ... CASP3(836)

安全信息

储存分类代码10 - Combustible liquids

Sigma-Aldrich

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