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CpG WIZTM E-Cadherin Amplification Kit

品牌
Sigma-Aldrich
货号
S7804
规格纯度
The CpG WIZ E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP).
参考价格
9446.8 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
CpG WIZTM E-Cadherin Amplification Kit
CD324
产品介绍:

产品说明

一般描述

E-cadherin is a cell adhesion protein that suppresses tumor cell invasion and metastasis in tumor models. On mechanism regulating the expression of the E-cadherin gene is methylation of the promoter region. The CpG WIZ E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and unmethylated) for E-cadherin are also included.



Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

应用

Principles of the Technique

Use of either the CpGenome DNA Modification Kit (Cat. No. S7820) or the CpGENOME Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ E-cadherin Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
The CpG WIZ E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP).

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
CPGWIZ is a trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32161000

产品性质

质量水平100
species reactivityhuman
manufacturer/tradenameCPGWIZ
Chemicon®
technique(s)PCR: suitable
NCBI登记号NM_004360.2
UniProt登记号P12830
application(s)genomic analysis
运输dry ice

安全信息

储存分类代码10 - Combustible liquids

Sigma-Aldrich

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