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位置:首页 > 品牌 > Sigma-Aldrich > Monoclonal Anti-Sonic Hedgehog (Shh) 小鼠抗

Anti-Sonic Hedgehog antibody, Mouse monoclonal

Monoclonal Anti-Sonic Hedgehog (Shh) 小鼠抗

品牌
Sigma-Aldrich
货号
S8321
规格纯度
clone SH154, purified from hybridoma cell culture
参考价格
6557.59 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-Sonic Hedgehog antibody, Mouse monoclonal
Anti-Shh
Monoclonal Anti-Sonic Hedgehog (Shh) 小鼠抗
Anti-TGN46
产品介绍:

产品说明

一般描述

The Hedgehog (Hh) family of secreted proteins consists of sonic hedgehog (Shh), desert hedgehog (Dhh) and Indian hedgehog (Ihh). The Hh signaling regulates cell migration and tissue construction during embryogenesis and reconstruction in adults. Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, γ catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system. Hedgehog signaling has been implicated in childhood tumors of pancreas, liver, thyroid, gastrointestinal tract, neuroblastomas and medulloblastomas
Anti-Sonic Hedgehog antibody recognizes human, rat and mouse Shh.

特异性

Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody recognizes human, rat, and mouse Shh.

应用

Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody may be used in ELISA, immunoblotting, and immunohistochemistry. It is used as a probe to determine the presence and roles of Sonic Hedgehog in processes such as patterning during embryogenesis.
Anti-Sonic Hedgehog antibody may be used for immunoblotting at a working concentration of ~2 μg/ml. A working antibody concentration of ~2 μg/ml is recommended for ELISA. For immunohistochemistry a dilution of 1:500 may be used.

生化/生理作用

Sonic Hedgehog (Shh) is an important cell signaling molecule expressed during embryonic development. Shh is involved in patterning of the developing embryonic systems such as the nervous system, somites, and limbs. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened. Shh protein is expressed in key embryonic tissues such as the Hensen′s node, zone of polarizing activity in the posterior limb bud, notochord, and floor plate of the neural tube. Downstream targets of Shh include the transcription factors Gli3, responsible for Greigs polycephalosyndactyly in humans, and Hoxd13, responsible for polysyndactyly.

外形

Solution in 0.01 M phosphte buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD06798717

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆SH154, monoclonal
形式buffered aqueous solution
species reactivityhuman, rat, mouse
浓度~2 mg/mL
technique(s)immunohistochemistry: 1:500 using formaldehyde-fixed human tissue sections
indirect ELISA: 2 μg/mL
western blot: 2 μg/mL using recombinant mouse SHH peptide
同位素/亚型IgG1
UniProt登记号Q15465
运输dry ice
储存温度−20℃
Gene Informationhuman ... SHH(6469)
mouse ... Shh(20423)
rat ... Shh(29499)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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