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Anti-Spred-2 antibody produced in rabbit

品牌
Sigma-Aldrich
货号
S7320
规格纯度
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
参考价格
7723.09 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti-Spred-2 antibody produced in rabbit
Anti-Sprouty Protein with EVH-1 Domain 2
产品介绍:

产品说明

一般描述

Spred proteins mainly function as inhibitors of the ERK and MAPK signaling cascades. Phosphorylation and Cbl-dependent ubiquitination regulate Spred-2 expression levels. Spred2 negatively modulates the hypothalamic-pituitary-adrenal axis . Anti-Spred-2 antibody is specific for human, rat, and mouse Spred-2 (approx. 48 kDa). Staining of the Spred-2 band in immunoblotting is specifically inhibited by the immunizing peptide.
Anti-Spred-2 is developed in rabbit using as immunogen a synthetic peptide corresponding to amino acids of mouse Spred-2, conjugated to KLH via an N terminal added cysteine residue. Spred-2, also known as sprouty protein with EVH-1 domain 2 related sequence, was isolated by similarity to Spred-1, which in turn was isolated from an osteoclast complementary DNA library by a two-hybrid system using c-Kit and c-Fms tyrosine kinase domains as bait. These proteins contain a carboxy-terminal cysteine-rich domain related to sprouty (the SPR domain), and an amino-terminal Ena/vasodilator stimulated phosphoprotein (VASP) homology (EVH-1) domain. This gene is mapped to human chromosome 2p14.

免疫原

synthetic peptide corresponding to amino acids 280-297 of mouse Spred 2. The sequence is conserved in rat and differs from human by three amino acids.

应用

Anti-Spred-2 antibody produced in rabbit has been used in western blotting and indirect immunofluorescence.

生化/生理作用

Spred inhibits growth factor mediated activation of MAP kinase. Spred inhibits the activation of MAP kinase probably by mechanisms such as suppression of phosphorylation and activation of Raf. Spred-2 functions as a negative regulator of AGM (aorta-gonad-mesonephros) hematopoiesis by inhibiting hematopoietic cytokine signaling. Spred proteins are also regulators of Rho-mediated cell motility.

外形

Solution in 0.01 M phoshate buffered saline, pH 7.4, 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD07784893
NACRESNA.44

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
分子量 ~48 kDa
species reactivityhuman, rat, mouse
浓度~1 mg/mL
technique(s)indirect immunofluorescence: 5-10 μg/mL using PC-12 cell lines
microarray: suitable
western blot: 1-2 μg/mL using cell extracts of HepG2
western blot: 2-4 μg/mL using mouse liver extracts
UniProt登记号Q7Z698
运输dry ice
储存温度−20℃
Gene Informationhuman ... SPRED2(200734)
mouse ... Spred2(114716)
rat ... Spred2(305539)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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