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Turbonuclease from Serratia marcescens

内切核酸酶 来源于粘质沙雷氏菌

品牌
Sigma-Aldrich
CAS
9025-65-4
货号
T4330
规格纯度
recombinant, expressed in E. coli
参考价格
4057.56 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
9025-65-4
Turbonuclease from Serratia marcescens
Endonuclease from Serratia marcescens
内切核酸酶 来源于粘质沙雷氏菌
产品介绍:

产品说明

一般描述

Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.

应用

Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.
Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating.
用于去除蛋白样品中的核酸。

生化/生理作用

Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA.
Endonuclease from Serratia marcescens is effective against both single- and double-stranded DNA and RNA. It mediates the digestion of the 3′ O—P bond resulting in oligonucleotides ending with 5′ monophosphate. The activity of this enzyme is known to be less affected by the reducing and chaotropic agents. It is highly stable at room temperature. This endonuclease eliminates the undesired nucleic acids in downstream processing.
消化天然或热变性的DNA和RNA。

单位定义

One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 ℃.

外形

Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl

基本信息

酶学委员会(EC)编号3.1.30.2 ( BRENDA | IUBMB )
MDL编号MFCD00131010

产品性质

质量水平200
重组expressed in E. coli
形式liquid
浓度≥200,000 units/mL
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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