产品说明
生化/生理作用
Tumor necrosis factor-β (TNF-β), also known as lymphotoxin-α (LT-α) and cytotoxic factor, is a 25 kDa glycoprotein that is tightly regulated by lymphocytes. TNF-β and TNF-α share a 28% amino acid sequence identity, and as soluble forms, both can exist in solution as homotrimers, activate the same TNF receptors (TNFR1 and TNFR2) and elicit many of the same bioactivities. Unlike TNF-α, TNF-β can also form heterotrimers with one or two molecules of the related membrane-bound lymphotoxin-β (LT-β). Reportedly, LTα1/β2, the predominant heterotrimer, binds and activates only the LT-β receptor, LT-βR. The other heterotrimer, LT-α2/β1 binds and activates TNFR1 and TNFR2 but not LT-βR. TNF-β is expressed in activated T, B and NK cells and in certain transformed cells. Genes for TNF-β, LT-β and TNF-α are tightly linked near each other within the MHC. TNF-β appears to play many of the same biological roles as TNF-α, but the membrane-bound form with LT-β is critical for normal lymphoid organ development. Genetic polymorphisms in TNF-α and TNF-β have been linked to certain pathological conditions, including myasthenia gravis. At the amino acid level, human and mouse TNF-β are 74% homologous and human and mouse LT-β are 80% homologous.
外形
lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein
分析说明
The biological activity is measured in a cytotoxicity assay using the TNF-suspectible murine L929 cell line in the presence of actinomycin D.
基本信息
MDL编号 | MFCD00163958 |
NACRES | NA.77 |
产品性质
质量水平 | 200 |
生物来源 | human |
重组 | expressed in E. coli |
测定 | >95% (SDS-PAGE) |
形式 | powder |
效能 | 4-20 pg/mL Activity |
质量 | endotoxin tested |
分子量 | protein 18.8 kDa |
包装 | pkg of 10 μg |
储存条件 | avoid repeated freeze/thaw cycles |
technique(s) | cell culture | mammalian: suitable |
杂质 | ≤1 EU/μg |
UniProt登记号 | P01374 |
储存温度 | −20℃ |
Gene Information | human ... LTA(4049), TNFSF13B(10673) |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich