产品说明
一般描述
Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.
特异性
识别 V5 标签(GKPIPNPLLGLDST)融合蛋白
应用
Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag. This tag facilitates the detection, isolation and purification of the proteins. Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
法律信息
Cy3 is a trademark of Cytiva
基本信息
| MDL编号 | MFCD03457579 |
| NACRES | NA.56 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | CY3 conjugate |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | V5-10, monoclonal |
| 浓度 | ~1 mg/mL |
| technique(s) | immunocytochemistry: 2.0-4.0 μg/mL |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
