产品说明
一般描述
Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.
特异性
Reactivity with other species has not been determined.
This antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells.
免疫原
Epitope: C-terminus
应用
Detect Tubulin β-III using this Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated validated for use in IC, IF, WB, IH & IP.
Immunohistochemistry: 5-10 µg/mL on frozen brain tissue.
Although this conjugated antibody will work for Western blotting and Immunoprecipitation, we recommend catalog number CBL412 if these are your primary applications.
Optimal working concentration must be determined by end user.
Research Sub Category
Neurofilament & Neuron Metabolism
Research Category
Neuroscience
质量
Routinely tested cortical neurons in Immunocytochemistry.
Immunocytochemical Analysis: 1:250 dilution of this lot detected β-III tubulin in rat cortex cell culture.
目标描述
50 kDa
外形
Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 6 months at 2-8°C from date of receipt.
分析说明
Control
Rat Cortex Cells.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
偶联物 | ALEXA FLUOR® 488 |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | TU-20, monoclonal |
species reactivity | bovine, primate, mouse, pig, human, rat |
technique(s) | immunocytochemistry: suitable immunofluorescence: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NP_001184110 |
UniProt登记号 | Q13509 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich