Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2

产品说明

一般描述

Sulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferase enzymes in the trans-Golgi-network. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosines in the total protein content of a cell can be sulfated. Sulfotyrosines mediates many receptor-ligand binding interactions, including the interaction of P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses; for example, tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells, and tyrosine sulfation of the Duffy antigen/receptor for chemokines is crucial for erythrocyte invasion by the malaria parasite Plasmodium vivax.
This clone was derived from the antibody described in Kehoe et al., 2006.

特异性

Recognizes sulfated tyrosine residues on various proteins. Does not recognize phosphotyrosine or unmodified tyrosines.
The antibody is predicted to recognize any sulfotyrosine, regardless of surrounding amino acid sequence.

免疫原

This antibody was developed using a phage display library as described in Kehoe et al., 2006. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC
Epitope: Sulfated Tyrosine

应用

Research Sub Category
General Post-translation Modification
Research Category
Signaling
WB, IP, ELISA
Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2 is a high quality Mouse Monoclonal Antibody for the detection of Sulfotyrosine & has been validated in WB, IP, ELISA.

质量

Assayed using sulfatase treated bovine fibrinogen vs. untreated fibrinogen.

目标描述

n/a

外形

100 μl in 100 mM Tris, 150 mM NaCl, 180 mM Glycine, pH 7.5 containing 0.05% sodium azide.
Format: Purified
Culture supernatant, purified by protein G affinity chromatography

储存及稳定性

Stable for 1 year from date of receipt when stored at +2-8°C.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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