Anti-MIXL1 Antibody

产品说明

一般描述

MIXL1 is also known as Homeobox protein MIXL1, Homeodomain protein MIX (hMix), MIX1 homeobox-like protein 1, and Mix.1 homeobox-like protein. MIXL1 is a transcription factor with roles in axial mesendoderm morphogenesis, endoderm formation, blood cell differentiation, and morphogenesis of the heart and the gut during embryogenesis. MIXL1 has been shown to negatively regulate brachyury expression. MIXL1 is expressed mainly in progenitors and secondary lymph tissues, immature B- and T-lymphoid cells during normal hematopoiesis, and is also present in differentiating embryonic stem cells.

特异性

Other homologies: Mouse and Rat (85% sequence homology).
This antibody recognizes the homeobox DNA binding domain of MIXL1.

免疫原

Epitope: Homeobox DNA binding domain
KLH-conjugated linear peptide corresponding to the homeobox DNA binding domain of human MIXL1.

应用

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected MIXL1 in H9 embryoid body cells. This protein was observed in H9 embryoid bodies (Lee, M. R., et al. (2010). Stem Cells. 28:1550-1559). MIXL1 was also localized in the nucleus of COS-7 and mouse ES cells (Mossman, A. K., et al. (2005). Stem Cells and Development. 14:656-663).
Anti-MIXL1 Antibody is a highly specific rabbit polyclonal antibody, that targets Homeobox protein MIXL1 & has been tested in western blotting & ICC.
Research Category
Signaling
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MIXL1 in 10 µg of Jurkat cell lysate.

目标描述

~36 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Jurkat cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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