产品说明
一般描述
Monoclonal Anti-ZAP-70 (mouse IgG1 isotype) is derived from the hybridoma 1E7.2 produced by the fusion of mouse myeloma cells and splenocytes from mice. ZAP-70 is a protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells.
免疫原
The immunogen sequence is conjugated to KLH.
peptide corresponding to amino acids 282-307 of human ZAP-70.
应用
Monoclonal Anti-ZAP-70 antibody has been used in
生化/生理作用
Zeta-chain-associated protein kinase70 (ZAP-70) is required for T-cell activation and is activated by(Tyrosine protein kinase) Lck-mediated phosphorylation of its tyrosine residues. Mutation of the gene that creates a defective ZAP-70 results in severe combined immunodeficiency syndrome (SCID).
ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the zeta chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling . Lack of ZAP-70 leads to an absence of antigen-specific response and also causes impaired T cell differentiation . Anti-ZAP-70 antibody is specific for human and mouse ZAP-70 (approx. 70 kDa).
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00803577 |
| NACRES | NA.44 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 1E7.2, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~70 kDa |
| species reactivity | mouse, human |
| 包装 | antibody small pack of 25 μL |
| 浓度 | ~1.5 mg/mL |
| technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 0.5-1.0 μg/mL using Jurkat cell extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P43403 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... ZAP70(7535) mouse ... Zap70(22637) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
