产品说明
特异性
MAB1371Z is reactive with mouse ICAM-1 (CD54), which is present on T-cells, B-cells, monocytes and endothelial cells, epithelial cells, dendritic cells and keratinocytes. Expression of CD54 is increased after stimulation with cytokines and LPS, and it also plays a role in B cell-T cell interactions.
应用
Research Category
Cell Structure
Anti-ICAM-1 Antibody, clone 3E2B, Azide Free detects level of ICAM-1 & has been published & validated for use in ELISA, FC, FUNC, IC, IH.
Immunocytochemistry: Live or acetone fixed cells
ELISA
Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested).
Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells.
Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary
Clone 3E2B is reported to be function blocking against murine ICAM-1.
Optimal working dilutions must be determined by end user.
Research Sub Category
Adhesion (CAMs)
联系
Replaces: CBL1331F
外形
Purified IgG prepared by affinity chromatography on Protein G agarose beads. Liquid at 1 mg/mL in sterile-filtered PBS, containing no preservatives. Endotoxin level 4.13EU/mg
Format: Purified
储存及稳定性
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | hamster (Armenian) |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 3E2B, monoclonal |
species reactivity | mouse |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_000201.1 |
UniProt登记号 | P05362 |
运输 | dry ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich