Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate

产品说明

一般描述

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

特异性

Recognizes Akt1/PKBα.

免疫原

GST-fusion protein corresponding to residues 1-149 of human Akt1

应用

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

质量

Evaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).

目标描述

~ 60 kDa observed

外形

Protein G Chromatography
20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.
Format: Purified

储存及稳定性

Stable for 1 year at 2-8ºC from date of receipt.

分析说明

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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